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Lab Photos |
More specific details will be added in the future, please see my Publications page for
current work.
Overview: The Pelling Lab is generally interested in understanding dynamic
mechanical properties of cellular systems across nanometer and
micrometer length scales. Utilizing a combination of cell biology,
molecular biology, physical and computational approaches we are
attempting to understand the fundamental genetic and architectural
control mechanisms of mechanotransduction pathways in cells and
artificial tissues.
Experimental Approaches: Atomic Force Microscopy (AFM);
Simultaneous AFM and Laser Scanning Confocal Microscopy (LSCM);
Fluorescence Recovery after Photobleaching (FRAP); Traction Force Microscopy (TFM); Particle Tracking; AFM based Rheology;
Computational Analysis (Image Registration, Tractions, Tracking, Signal
Processing, Fourier/Correlation Analysis/Spectroscopy and Mechanical
Analysis); Cell and tissue culture (human and animal cell lines); Molecular Biology (Bacteria, RNAi,
Fusion Proteins)
Projects
Cytoskeletal deformation, shock absorption and force
dissipation in 3D
Nuclear shape and volume anisotropy during active and
passive 3D deformations
Emergent nanomechanical properties in simple artificial
tissues created from normal and genetically modified precursor cells
pH variations during dynamic loading in cellular micro/mechano-environments
Cardiomyocyte mechano, traction and fluid dynamics
Mechanical dynamics during myogenesis
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